Analysis Note

ControlA431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ASS1 in human cervical cancer and colon cancer tissues.

Optimal working dilutions must be determined by end user.

Detect Argininosuccinate synthase using this mouse monoclonal antibody, Anti-ASS1 Antibody, clone 2C10 validated for use in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Argininosuccinate synthase (ASS1) or alternatively known by the older name of Citrulline-Aspartate ligase is encoded by the ASS1/ASS gene in humans and is the enzyme that is responsible for the third step in the urea cycle critical to the metabolism of nitrogen. ASS1 catalyzes the synthesis of argininosuccinate from citrulline and aspartate. Argininosuccinate is the immediate precursor of arginine and ASS1 was first identified as the rate limiting step in the liver for this reaction. ASS1 is now recognized to be expressed ubiquitously in mammalian tissues. ASS1 also performs the rate limiting step for NO production in the citrulline-NO cycle as well. Regulation of ASS1 expression can vary depending upon the tissue examined. In the liver, where arginine is hydrolyzed to form urea and ornithine, ASS1 is highly expressed, and various hormones such as glucocorticoids, glucagon, insulin and even high dietary levels of specific amino acids like glutamine regulate expression of ASS1 during development and adult life. In other tissues and in particular in nitric oxide (NO) producing cells, ASS1 is expressed at a low level but induced by proinflammatory cytokines. Interestingly ASS1 expression is also increased in Alzheimer's disease in both neurons and glia, which also is correlated with iNOS expression and suggests a link between increased NO production and iNOS expression observed in Alzheimer brains. EMD-Millipore's Anti-ASS1 monoclonal antibody has been tested in western blot on recombinant protein and cell lysates from A431, Raji, MOLT4, Jurkat, A549, NIH3T3, PC-12, and COS7 and in paraffin embedded immunohistochemistry on cervical cancer tissue sections. The antibody has also been tested in direct ELISA on recombinant antigen.

Immunogen

Purified recombinant fragment of human ASS1 expressed in E. Coli.

Physical form

Protein G Purified

Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Format: Purified

Quality

Evaluated by Western Blotting in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ASS1 in A431, Raji, MOLT4, Jurkat, A549, NIH/3T3, PC-12 and Cos7 cell lysates.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~47 kDa observed. Uncharacterized bands may appear in some lysate(s).